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Benoit Barbeau, PhD

Professor
Université du Québec à Montréal
Sciences biologiques
2080 St-Urbain, local SB-3335
Montréal (Québec)  H2X 3X8
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Phone : 514 937-3000 #4576                       
Fax: 514 987-4647

Field of research:

Our research interests are focussed on human retroviruses, such as HIV-1 and HTLV viruses. More recently, we also became interested in Human Endogenous Retroviruses and their implication in the formation of the human placenta. We are currently conducting a series of studies aimed at the understanding of their role in placental functions and we are particularly concentrating our efforts on their association to obstetrical disorders, such as preeclampsia.

Recent results:

Our research team has recently initiated studies on placenta-derived exosomes. We have shown that, in pregnant women, these circulating microvesicles harboured Syncytin-1 and Syncytin-2, two Human Endogenous Retrovirus-derived proteins, on their surface. Alike for ancestral viruses, these proteins seem to provide a tropism to placental exosomes, and are thereby targeting their internalization by specific cell types expressing appropriate receptors on their surface. In addition, our results have suggested that Syncytin-2 levels are lower in serum-derived exosomes from preeclamptic women than in exosomes from normal pregnant women. These latter results argue for the potential future development of an early diagnostic tool for preeclampsia predisposition through the detection of Syncytin-2 in serum exosomes.

Laboratory members:

Name

Position

Email address

Clément Caté

PhD Student

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Xavier Duthoit

MSc Student

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Kevin Gayout

Technician

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Zhenlong Liu

PhD Student

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Gatien Lokossou

Post-doctoral Trainee

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Caroline Toudic

PhD Student

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Yong Xiao

Research Agent

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Mellisha Richard Dawe

MSc Student

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Publications:

  1. Larguet, F., Barbeau, B., Rassart, É and Edouard, E. (2015) HDAC1 interacts with HIV-1 integrase and modulates virus replication. Virology (submitted).
  2. Hubert, A. Barbeau, B., Subra, C., Bissonnette, L., and Gilbert, C. (2015) Role and future application of extracellular vesicles in HIV-1 pathogenesis, Future Virol. (in press).
  3. Toufaily, C., Vargas, A., Rassart, É., and Barbeau, B. (2015) Identification of a CREB/ATF binding site in the Syncytin-2 promoter essential for its activity. PLoS One 10: e0121468
  4. Barbeau, B. and Mesnard, J.M. (2015) Review antisense transcription in retroviruses. Trends in Microbiology Feb 17 [Epub ahead of print].
  5. Torresilla, C. Mesnard, J.M., and Barbeau, B. (2015) An old viral gene revisited: the HIV-1 antisense protein Current HIV Research Dec 2 [Epub ahead of print].
  6. Lokossou, G., Toudic, C., and Barbeau, B. (2014) Implication of Human Endogenous Retrovirus Envelope proteins in placental functions. Viruses 6: 4609-4627.
  7. da Conceição AO, von Poser GL, Barbeau B, Lafond J. (2014) Hypericum caprifoliatum and Hypericum connatum affect human trophoblast-like cells differentiation and Ca(2+) influx. Asian Pac J Trop Biomed. 4:367-73.
  8. Arpin-André, C., Laverdure, S., Barbeau, B. Gross, A., and Mesnard, J.M. (2014) Construction of a reporter vector for analysis of bidirectional transcriptional activity of retrovirus LTR. Plasmid 74: 45-51.
  9. Vargas, A., Éthier-Chiasson, M., Flipo, D., :Lafond, J., Gilbert, C., and Barbeau, B. (2014) Human Endogenous Retrovirus envelopes incorporated in retroviral-like placenta exosomes are important for cell uptake and are potential preeclampsia biomarkers. FASEB J. May 8 [Epub ahead of print].
  10. Larocque, É., Halin, M., Landry, S., Switzer. W., Mesnard, J.M., Lemay, G., and Barbeau, B. (2014) HTLV-3 and HTLV-4 antisense proteins activate Jun-B-, c-Jun- and Jun-D-dependent transcription. J. Virol. 88:8956-70.
  11. Barbeau, B., Hiscott, J., Bazarbachi, A., Carvalho, E., Jones, K., Martin, F., Matsuoka, M., Murphy, E., Ratner, L., Switzer, W. and Watanabe, T. (2014) Conference highlights of the 16th international conference on human retrovirology: HTLV and related retroviruses, June 26-30th 2013 Montreal, Canada. Retrovirology 11: 19. Highly accessed
  12. Barbeau, B., Peloponese, J.M., and Mesnard, J.M. (2013) Functional comparison of the antisense proteins of HTLV-1 and HTLV-2 in viral pathogenesis. Frontiers in Microbiology 4: 1-8
  13. Torresilla, C., Larocque, E., Landry, S., Halin, M., Coulombe, Y., Masson, J.Y., Mesnard, J.M., and Barbeau, B. (2013) Expression and detection of HIV-1 ASP derived from the minus strand of HIV-1 and its implication in autophagy. J. Virol. 89: 5089-5105.
  14. Toufaily, C., Vargas, A., Lemire, M., Rassart, É., and Barbeau, B. (2013) MFSD2a, the Syncytin-2 receptor, is important for trophoblast fusion. Placenta 34: 85-88.
  15. Laverdure, S., Gross, A., André-Arpin, C., Clerc, I., Beaumel, B., Barbeau, B., and Mesnard, J.M. (2012) HIV-1 antisense transcription is preferentially activated in primary monocyte-derived cells. J. Virol. 86:13785-13789.
  16. Tolosa, J.M., Schjenken, R., Clifton, V., Vargas, A., Barbeau, B., Lowry, P., Maiti, K., and Smith, R.   (2012) Human placental exosomes carry the immunosuppressive retroviral envelope protein syncytin-1. Placenta 33: 933-941
  17. Gazon, H., Lemasson, I., Polakowski, N., Césaire, R., Matsuoka, M., Barbeau, B., Mesnard, J.M., and Peloponese, J.M., (2012) Human T-cell Leukemia Virus type 1 (HTLV-1) bZIP factor requires cellular transcription factor JunD to upregulated HTLV-1 antisense transcription for the 3’ LTR. J. Virol. 86: 9070-9078.
  18. Audet, B., Torresilla, C., Robichaud, G.A., Tremblay, M.J., and Barbeau, B. (2012) Molecular mechanism leading to preferential replication pf HIV-1 in memory CD4+ T lymphocytes. IN Tyrosine: properties, sources and health benefits (invited chapter), Nova Science Publishers, Inc. Hauppauge NY.
  19. Vargas, A., Larocque, É., Toufaily, C., Rassart, É., Lafond, J., and Barbeau, B. (2012) Quantitative assay of trophoblast fusion through a luciferase reporter gene under the regulation of the HIV-1 LTR. Reproductive Sciences 19: 374-382.
  20. Vargas, A., Thiery, M., Lafond, J., and Barbeau, B. (2012) Transcriptional and functional studies of the HERV EnvV and EnvP(b) proteins in human trophoblasts. Virology 425: 1-10.
  21. Toufaily, C., Landry, S., Leib-Mosch, C., Rassart, E., and Barbeau, B. (2011) Activation of LTRs from different HERV families by the HTLV-1 Tax protein and T-cell activators. Viruses 3(11):2146-59
  22. Larocque, É., Halin, M., Landry, S., Marriott, S., Switzer, W.M., and Barbeau, B. (2011) HTLV-3 and HTLV-4 derived antisense transcripts encore proteins with similar Tax-inhibiting function but distinct subcellular localization. J. Virol. 85:12673-85.
  23. Vargas, A., Toufaïly, C., LeBellego, F., Rassart, É., Lafond, J., and Barbeau, B. (2011) Reduced expression of both Syncytin-1 and Syncytin-2 correlates with severity of pre-eclampsia. Reproductive Sciences 18: 1085-1091.
  24. Clerc, I., Laverdure, S., Torresilla, C., Landry, S., Borel, S., Arpin-André, C., Gay, B., Briant, L., Gross, A., Barbeau**, B., and Mesnard**, J.-M. (2011) Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells. Retrovirology 8: 74-86. Highly accessed.
  25. Barbeau, B., and Mesnard, J.M. (2011) What sense can we make out of antisense transcription in Human T-cell lymphotropic viruses (HTLVs)? Viruses (special issue) 3: 456-468.
  26. Haché, S., Takser, L., LeBellego, F., Weiler, H., Leduc, L., Forest, J.C., Giguère, Y., Masse, A., Barbeau, B. and Lafond, J. (2011) Alteration of placental calcium homeostasis in preeclampsia: effect on calcium exchange in placenta. J. Cell. Mol. Med.15: 654-667.
  27. Barbeau, B., Devaux, C., and Mesnard, J.-M. (2010) Antisense transcription in Human T-cell Leukemia Virus type 1: discovery of a new viral gene. In Human retroviruses, Woled Scientific, NJ
  28. Duc Dodon, M., Barbeau, B., and Mesnard, J.-M. (2010) La leucémie de type T chez l’adulte induite par HTLV-1: y a-t-il un avant et un après HBZ? Médecine/Sciences 26: 391-396.

** co-corresponding authors

Awards:

Chaire de recherche du Canada en rétrovirologie humaine, niveau 2 (2005-2015)