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   CPrice
    

Christopher A. Price, Ph.D

Professor

Head, Department of Veterinary Biomedicine

Université de Montréal

Faculté de médecine vétérinaire, CRRF

3200 Sicotte

St-Hyacinthe, Qc

J2S 2M2 Canada

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Phone: 450 773-8521  ext. 8383   

Fax: 450 778-8103

 

Research Interest:

  • Control of follicle development

 

Summary of Results:

The overall objective of my laboratory is to understand better the regulation of follicle cell health and survival. During follicle development, there is significant growth of the follicle and proliferation and differentiation of granulosa and of theca cells.  We are currently studying roles of members of the fibroblast growth factor (FGF) family. This large family contains 22 peptides and 7 receptors (which result from alternative splicing of 4 FGFR genes).  We have described the expression of a number of these FGFR splice variants in the bovine follicle, and we are determining which FGFs play important roles in follicle development. We have shown that FGF10 mRNA and protein are present in theca cells of the follicle, and expression  is higher in atretic/regressing follicles. As FGF10 significantly inhibits estradiol secretion from granulosa cells, we propose that FGF10 is involved in the regulation of follicle growth.  

We have extending this work to look at another FGF, FGF18. We have detected FGF18 mainly in theca cells of atretic follicles. In vitro experiments demonstrate that FGF18 causes granulosa cell death by apoptosis, and that this is estradiol-dependent. Curiously, FGF18 does not activate the typical FGF signaling pathways; we are currently working to identify how FGF18 affects follicle growth.

We also have an applied project underway to study the effect of fungal toxins on ovarian function, in collaboration with Dr Chorfi (RQR) and a veterinary embryo transfer company (MediVet Inc). We have demonstrated that deoxynivalenol, a common contaminant of animal feedstuff, increases apoptosis in granulosa and theca cells. We are now exploring the mechanism of action of these toxins.

Lab Members:

Name

Position

Email address

Anthony Estienne

Étudiant Postdoc

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Lauriane Relav Étudiant PhD This email address is being protected from spambots. You need JavaScript enabled to view it.
Morad Ben Koura Étudiant MSc This email address is being protected from spambots. You need JavaScript enabled to view it.

 

List of Publications (selected):

Soares ACS, Lodde V, Barros RG, Price CA, Luciano AM, Buratini J (2017) Steroid hormones interact with natriuretic prptide C to delay nuclear maturation, to maintain oocyte-cumulus communication and to improve the quality of in vitro-produced embryos in cattle. Reprod Fertil Develop in press.

Lima PF, Ormond CM, Caixeta ES, Barros RG, Price CA, Buratini J. (2016) Effect of kit ligand on natriuretic peptide precursor C and oocyte maturation in cattle. Reproduction 152: 481-489.

Jaton C, Koeck A, Sargolzaei M, Price C, Baes C, Schenkel F, Miglior F. (2016) Short communication: Genetic correlations between number of embryos produced using in vivo and in vitro techniques in heifer and cow donors. J Dairy Sci. 99:8222-8226.

Portela VM, Castilho AC, Bertolin K, Buratini J, Price CA (2016) Localization of angiotensin receptor type 2 in fetal bovine ovaries. Animal Reproduction Science 168: 34-39.

Jaton C, Koeck A, Miglior F, Price CA, Sargolzaei M & Schenkel FS. (2016) Genetic analysis of superovulatory response of Holstein cows in Canada. J Dairy Sci 99:3612-3623.

Abedini A, Zamberlam G, Lapointe E, Tourigny C, Boyer A, Paquet M, Hayashi K, Honda H, Kikuchi A, Price C, Boerboom D. (2016) WNT5a is required for normal ovarian follicle development and antagonizes gonadotropin responsiveness in granulosa cells by suppressing canonical WNT signaling. FASEB Journal 30:1534-1547.

Price CA (2016) Invited review: Mechanisms of fibroblast growth factor signaling in the ovarian follicle. Journal of Endocrinology 228: R31-R43.

Castilho ACS, Price CA, Dalanezi F, Ereno RL, Machado MF, Barros CM, Gasperin BG, Gonçalves PBD, Buratini J (2015) Evidence that fibroblast growth factor 10 plays a role in follicle selection in cattle. Reproduction, Fertility and Development in press.

de Souza LB, Paradis M, Zamberlam G, Benoit-Biancamano MO, Price C (2015) Identification of 5α-reductase isoenzymes in canine skin. Veterinary Dermatology 26: 363-e81.

Machado MF, Caixeta ES, Sudiman J, Gilchrist RB, Thompson JG, Lima PF, Price CA, Buratini J. (2015) Fibroblast growth factor 17 and bone morphogenetic protein 15 enhance cumulus expansion and improve quality of in vitro-produced embryos in cattle. Theriogenology 84: 390-398.

Guerrero-Netro HM, Chorfi Y, Price CA (2015) Effects of the mycotoxin deoxynivalenol on steroidogenesis and apoptosis in granulosa cells. Reproduction 149: 555-561.

Abedini A, Zamberlam, G, Boerboom D, Price CA. (2015) Non-canonical WNT5A is a potential regulator of granulosa cell function in cattle. Mol Cell Endo 403: 39-45.

Yapura J, Badea I, Zamberlam G, Price C, Mapletoft R, Pierson R, Singh J, Adams G. (2015) Formulation and testing of a non-steroidal aromatase inhibitor intravaginal device for the control of ovarian function in cattle. Animal Reproduction Science 156: 91-102.

Portela VM, Dirandeh E, Guerrero-Netro HM, Zamberlam G, Barreta MH, Goetten AF, Price CA (2015) The role of fibroblast growth factor-18 in follicular atresia in cattle. Biol Reprod. 92:14, 1-8.