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      richard      

François J. Richard, Ph.D.

Professor

Département Sciences animales

Faculté des sciences de l'agriculture et de l'alimentation

Université Laval

Pavillon Paul-Comtois, local 2781

Québec (Québec) G1K 7P4

Phone: (418) 656-2131, poste 2781

Fax: (418) 656-3766

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Research Interests: 

  • Oocyte
  • Maturation
  • IVF
  • Phosphodiesterase
  • Gap junction communication
  • Spermatozoa
  • Cumulus

 

Summary of Results:

My lab is interested in cellular signaling. We are studying phosphodiesterases in oocyte physiology, especially during oocyte maturation. We have also developed a system to measure gap junction communication live between cumulus cells in 3D. We are also interested in the study of phosphodiesterases in sperm function. Thus, we are developing a new culture media to improve oocytes quality in order to improve blastocysts rates in vitro produced.

 

Laboratory members:

Name

Position

Email Address

Marie-Pier Poulin

MSc student

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Amel Lounas

MSc student

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List of Publications:

Richard FJ, Santiquet N, Bergeron A, Khan DR. (2017) Signal transduction pathways in oocyte maturation. In Animal Models and Human Reproduction. First Edition. Edited by Heide Schatten and Gheorghe M Constantinescu. John Wiley & Sons Inc. 177.

Bergeron, A. Hébert, A. Guillemette, C. Laroche, A. Poulin, M-P. Aragon, J.P. Leclerc, P. Sullivan, R., Blondin, P. Vigneault, C. Richard, F.J.(2017). Papaverine-sensitive phosphodiesterase activity is measured in bovine spermatozoa. Andrology. 5(1): 169-179.

Marechal L, Guillemette C, Goupil S, Blondin P, Leclerc P, Richard FJ.(2017). Cyclic nucleotide phosphodiesterases in human spermatozoa andseminal fluid: Presence of an active PDE10A in human spermatozoa. Biochim Biophys Acta. 1861(2): 147-156.

Bergeron A, Aragon JP, Guillemette C, Hebert A, Sullivan R, Blondin P, Richard FJ.(2016). Characterization of cAMP-phosphodiesterase activity in bovine seminal plasma. Andrology. 4(6):1123-1130.

Macaulay, A. D. Gilbert, I. Scantland, S. Fournier, E. Ashkar, F. Bastien, A. Shojaei Saadi, H. A. Gagne, D. Sirard, M. A. Khandjian, E. W. Richard, F. J. Hyttel, P. Robert, C.(2016). Cumulus Cell Transcripts Transit to the Bovine Oocyte in Preparation for Maturation. Biol Reprod. 94(1): 16.

Gilchrist, R. B. Luciano, A. M. Richani, D. Zeng, H. Wang, X. De Vos, M. Sugimura, S. Smitz, J. Richard, F. J. Thompson, J. G.(2016). Oocyte maturation and quality: role of cyclic nucleotides. Reproduction. 152(5):R143-157.

Bergeron, A. Guillemette, C. Sirard, M. A. Richard, F. J.(2016). Active 3'-5' cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cells. Reprod Fertil Dev.

Goupil, S. Marechal, L. El Hajj, H. Tremblay, M. E. Richard, F. J. Leclerc, P.(2016). Identification and Localization of the Cyclic Nucleotide Phosphodiesterase 10A in Bovine Testis and Mature Spermatozoa. PLoS One. 11(8): e0161035.

Khan, D. R. Fournier, E. Dufort, I. Richard, F. J. Singh, J. Sirard, M. A.(2016). Meta-analysis of gene expression profiles in granulosa cells during folliculogenesis. Reproduction. 151(6): R103-11.

Gagnon, A. Khan, D. R. Sirard, M. A. Girard, C. L. Laforest, J. P. Richard, F. J.(2015). Effects of intramuscular administration of folic acid and vitamin B on granulosa cells gene expression in postpartum dairy cows. J Dairy Sci. 98(11): 7797-809.

Khan, D. R. Guillemette, C. Sirard, M. A. Richard, F. J.(2015). Characterization of FSH signalling networks in bovine cumulus cells: a perspective on oocyte competence acquisition. Mol Hum Reprod. 21(9): 688-701.

Khan, D. R. Guillemette, C. Sirard, M. A. Richard, F. J.(2015). Transcriptomic analysis of cyclic AMP response in bovine cumulus cells. Physiol Genomics. 47(9): 432-42.

Santiquet, N. Sasseville, M. Laforest, M. Guillemette, C. Gilchrist, R. B. Richard, F. J.(2014). Activation of 5' adenosine monophosphate-activated protein kinase blocks cumulus cell expansion through inhibition of protein synthesis during in vitro maturation in Swine. Biol Reprod. 91(2): 51.

Bunel A , Nivet AL , Blondin P , Vigneault C , Richard FJ , Sirard MA. (2014). Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes. Reprod Fertil Dev. 26(6): 855-865.

Macaulay, A. D. Gilbert, I. Caballero, J. Barreto, R. Fournier, E. Tossou, P. Sirard, M. A. Clarke, H. J. Khandjian, E. W. Richard, F. J. Hyttel, P. Robert, C.(2014). The gametic synapse: RNA transfer to the bovine oocyte. Biol Reprod. 91(4): 90.

Dallaire, M. P. Taga, H. Ma, L. Corl, B. A. Gervais, R. Lebeuf, Y. Richard, F. J. Chouinard, P. Y.(2014). Effects of abomasal infusion of conjugated linoleic acids, Sterculia foetida oil, and fish oil on production performance and the extent of fatty acid Delta(9)-desaturation in dairy cows. J Dairy Sci. 97(10): 6411-25.

Santiquet, N. Papillon-Dion, E. Djender, N. Guillemette, C. Richard, F. J.(2014). New elements in the C type natriuretic peptide signaling pathway inhibiting swine in vitro oocyte meiotic resumption. Biol Reprod. 91(1): 16.

Santiquet NW, Robert C, Richard FJ. The dynamics of connexin expression, degradation and localisation are regulated by gonadotropins during the early stages of in vitro maturation of swine oocytes. PlosONE 2013 8: e68456

Santiquet NW, Develle Y, Laroche A, Robert C, Richard FJ. Regulation of Gap Junctional Communication Between Cumulus Cells During In Vitro Maturation in Swine, a Gap-FRAP Study. Biol Reprod 2012 87:1-8.

Santiquet N, Vallieres L, Pothier F, Sirard MA, Robert C, Richard F. Transplanted bone marrow cells do not provide new oocytes but rescue fertility in female mice following treatment with chemotherapeutic agents. Cell Reprogram 2012; 14:123-129