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     nagano     

Makoto Nagano, PhD, DVM 

Associate Professor

McGill University    

Department: Obstetrics and Gynecology

Royal Victoria Hospital, Rm # F3.07

687 Pine Avenue West, Montreal, Quebec H3A 1A1

E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.   

Telephone: 514-934-1934 ext. 35250

Fax: 514-843-1662

 

Field of research:  

Biology and Clinical Applications of Male Germ Line Stem Cells

 

Recent results:

Our research interests are two-fold, surrounding male germ line stem cells, or spermatogonial stem cells. First, we want to understand the mechanism of stem cell self-renewal and commitment to differentiation using the mouse as a model species. In particular, we focus on what initiates commitment of stem cells into differentiation, what determines the “point-of-no-return” during the stem cell commitment, and what occurs in the course of the commitment process. In past years, we built great resources of biological/bioinformatics information and techniques necessary to tackle these objectives, including physiological and immunophenotypic changes during stem cell self-renewal and differentiation and how they behave when exposed to certain differentiation-inducing conditions. Based on our past accomplishments and data derived in other laboratories, we want to nail down the mechanism of stem cell commitment.

The second issue is to understand the biology of human male germ line stem cells and apply the acquired knowledge into clinical situations, particularly for establishing stem cell-based strategies for male fertility restoration in cancer survivors. We want to determine stem cell markers, induce stem cell self-renewal and proliferation, and manipulate stem cell commitment. With our clinical colleagues, we further aim at establishing the scheme by which we can isolate stem cells and eliminate tumorigenic cells from human testis cells in order to preserve and restore male fertility in cancer survivors.

We are constantly looking for enthusiastic and industrial trainees with whom we tackle these important objectives.

 

Laboratory members: 

Name

Position

Email Address

Xiangfan Zhang

Research Assistant

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Joëlle Desmarais

Postdoctoral fellow

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Ching-Wen Chang

Postdoctoral fellow

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Liang Ning

Visiting scholar

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MNagano

List of Publications: 

  1. *Yeh, J.R., *Zhang, X., Nagano, M.C. (2011) Wnt5a is a cell-extrinsic factor that supports mouse spermatogonial stem cell self-renewal. J. Cell Sci. 124: 2357-2366.
  2. *Zohni, K., *Zhang, X., Nagano, M.C. (2011) The efficiency of male fertility restoration is dependent on the recovery kinetics of spermatogonial stem cells after cytotoxic treatment with busulfan in mice. Hum. Reprod. Nov 14 Epub ahead of print
  3. *Ebata, K.E., *Yeh, J.R., *Zhang, X., and Nagano M.C. (2011) Soluble growth factors stimulate the division of spermatogonial stem cells that maintains a stem cell pool and produces progenitors in vitro. Exp. Cell Res. 317: 1319-1329.
  4. Nagano, M.C. (2011) Techniques for culturing spermatogonial stem cells continue to improve. Biol Reprod. 2011; 84: 5 – 6.        
  5. *Paul, C., Nagano, M., Robaire, B. (2011) Aging results in differential regulation of DNA repair pathways in pachytene spermatocytes in the Brown Norway rats. Biol. Reprod. 85: 1269-1278.
  6. *Marcon, L., *Zhang, X., Hales, B., Robaire, B., and Nagano, M.C. (2011) Impact of chronic exposure to chemotherapeutic agents for testiscular cancer on rat spermatogonial stem cells. J Androl. 32; 432-443.
  7. Nagano, M.C. and *Yeh, J.R. (2011) The cluster-forming activity (CFA) assay: A short-term in vitro method to detect mouse spermatogonial stem cells (Chapter 7). In: Male Germline Stem Cells: Developmental and Regenerative Potential (Eds: Orwig, K.E. and Harmann, B.). The Springer/Humana Press, New York, NY.   
  8. Boyer, A., *Yeh, J.R., *Zhang, X., Paquet, M., Gaudin, A., Nagano, M.C., Boerboom, D. CTNB1 signaling in Sertoli cells downregulates spermatogonial stem cell activity via Wnt4. PLoS One in press.
  9. *Marcon, L., *Zhang, X., Hales, B., Nagano, M.C., and Robaire, B. (2010) Development of a short-term fluorescence-based assay to assess the toxicity of anticancer drugs on rat spermatogonial stem cells in vitro. Biol Reprod 83:228-237.

 

Awards :

Nagano – FRSQ, Bourses de chercheurs boursiers, Fondamental

Jonathan Yeh – MUHC-RI studentship, International Society of Stem Cell Research Travel award (to Barcelona, Spain), Centre for Study of Reproduction studentship.

Highlight Articles in Biology of Reproduction – 2006 & 2007