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       M A Sirard4a
         

Marc-André Sirard, DMV, Ph.D.

Chaire de recherche du Canada Sénior en Génomique Animale

Université Laval

Département des Sciences Animales Pavillon des services, 

INAF, bureau 2732 Université Laval, Québec,

Canada, G1V 0A6

Tel : 418 656-7359

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.

http://www.crbr.ulaval.ca/

http://www.inaf.ulaval.ca/

http://www.reprogenomique.fsaa.ulaval.ca/

Research Interests:

  • Ocyte
  • Ovary
  • Biotechnologies
  • enomics
  • Ethic

 

Summary of Results:

Translation to come

Nos projets actuels sont:  

1) Épigénétique:comment l’ovule et le spermatozoïde transmettent l’influence de l’environnement

2) La compétence au développement de l'ovule

a) Par l'étude de la régulation des ARN maternels et des micro-ARN

b) Par l'étude des conditions folliculaires qui induisent la compétence ovocytaire

 

Lab Members:

Name

Position

Email Address

Florence Pagé-Larivière

Étudiante Ph.D

 This email address is being protected from spambots. You need JavaScript enabled to view it.

David Landry

Étudiant Ph.D. This email address is being protected from spambots. You need JavaScript enabled to view it.
Meihong Shi Étudiante Ph.D. This email address is being protected from spambots. You need JavaScript enabled to view it.
Daulat Khan Post-Doc

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Chongyang WU Étudiant Ph.D. This email address is being protected from spambots. You need JavaScript enabled to view it.
Isabelle Dufort Professionnel de recherche

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Chloé Fortin Étudiante M.Sc. This email address is being protected from spambots. You need JavaScript enabled to view it.
Léonie Morin Étudiante M.Sc. This email address is being protected from spambots. You need JavaScript enabled to view it.
Catherine Chaput Étudiante M.Sc. This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it. 

 

 

List of Publications (2013-2015):

Tremblay PG, SIRARD MA. Transcriptomic analysis of gene cascades involved in protein kinase A and C signalling in the KGN line of human ovarian granulosa tumour cells. Biol Reprod. 2017 Apr 5. doi: 10.1093/biolre/iox024. [Epub ahead of print] PubMed PMID: 28383663.

SIRARD MA. The influence of in vitro fertilization and embryo culture on the embryo epigenetic constituents and the possible consequences in the bovine model. J Dev Orig Health Dis. 2017 Mar 6:1-7. [Epub ahead of print] PubMed PMID: 28260557.

Morin-Doré L, Blondin P, Vigneault C, Grand FX, Labrecque R, SIRARD MA. Transcriptomic evaluation of bovine blastocysts obtained from peri-pubertal oocyte donors. Theriogenology. 2017 Apr 15;93:111-123.   PubMed PMID: 28257859.

Orozco-Lucero E, Dufort I, SIRARD MA. Regulation of ATF1 and ATF2 transcripts by sequences in their 3' untranslated region in cleavage-stage cattle embryos. Mol Reprod Dev. 2017 Feb 15. [Epub ahead of print] PubMed PMID: 28198054.

Vachon, J, Campagna,C, Rodriguez, M, SIRARD MA, Levallois, P. Barriers to the use of toxicogenomics data in human health risk assessment: A survey Regulatory Toxicology and Pharmacology 2017 Jan 27. (17)30008-9.PubMed PMID: 28137640.

Pagé-Larivière F, Campagna C, SIRARD MA. Mechanisms involved in porcine early embryo survival following ethanol exposure. Toxicol Sci. 2017 Jan 9. p256. PubMed PMID:28069986.

Desmet KL, Van Hoeck V, Gagné D, Fournier E, Thakur A, O'Doherty AM, Walsh CP, SIRARD MA, Bols PE, Leroy JL. Exposure of bovine oocytes and embryos to elevated non-esterified fatty acid concentrations: integration of epigenetic and transcriptomic signatures in resultant blastocysts. BMC Genomics. 2016 Dec8;17(1):1004. PMID: 27931182.

Pagé-Larivière F, Tremblay A, Campagna C, Rodriguez MJ, SIRARD MA. Low concentrations of bromodichloromethane induce a toxicogenomic response in porcine embryos in vitro. Reprod Toxicol. 2016 Sep 23. pii: S0890-6238(16)30344-6. PMID: 27671623.

Cagnone GL, SIRARD MA. The embryonic stress response to in vitro culture: Insight from genomic analysis. Reproduction. 2016 Dec;152(6):R247-R261. Review. PMID: 27601713.

Khan DR, Landry DA, Fournier É, Vigneault C, Blondin P, SIRARD MA. Transcriptome meta-analysis of three follicular compartments and its correlation with ovarian follicle maturity and oocyte developmental competence in cows. Physiol Genomics. 2016 Aug1;48(8):633-43. Epub 2016 Jul 8. PMID: 27401219.

Dieci C, Lodde V, Labreque R, Dufort I, Tessaro I, SIRARD MA, Luciano AM. Differences in cumulus cell gene expression indicate the benefit of a pre-maturation step to improve in-vitro bovine embryo production. Mol Hum Reprod. 2016 Dec;22(12):882-897. PMID: 27559149.

Shojaei Saadi HA, Gagné D, Fournier É, Baldoceda Baldeon LM, SIRARD MA, Robert C. Responses of bovine early embryos to S-adenosyl methionine supplementation in culture. Epigenomics. 2016 Aug;8(8):1039-60 PMID: 27419740.

Macaulay AD, Gilbert I, Scantland S, Fournier E, Ashkar F, Bastien A, Saadi HA, Gagné D, SIRARD MA, Khandjian ÉW, Richard FJ, Hyttel P, Robert C. Cumulus Cell Transcripts Transit to the Bovine Oocyte in Preparation for Maturation. Biol Reprod. 2016; 94(1):16 PMID: 26586844

Landry DA, Bellefleur AM, Labrecque R, Grand FX, Vigneault C, Blondin P, SIRARD MA. Effect of cow age on the in vitro developmental competence of oocytes obtained after FSH stimulation and coasting treatments. Theriogenology. 2016. 86(5):1240-6 PMID: 27215669.

SIRARD MA. Somatic environment and germinal differentiation in antral follicle: The effect of FSH with drawal and basal LH on oocyte competence acquisition in cattle. Theriogenology. 2016 86(1):54-61. Review. PMID: 27158126

Labrecque R, Fournier E, SIRARD MA. Transcriptome analysis of bovine oocytes from distinct follicle sizes: Insights from correlation network analysis. Mol Reprod Dev. 2016 ;83(6):558-69 PMID: 27127921.

Nivet AL, Léveillé MC, Leader A, SIRARD MA. Transcriptional characteristics of different sized follicles in relation to embryo-transferability: potential role of hepatocyte growth factor signalling. Mol Hum Reprod. 2016 Jul;22(7):475-84 PMID: 27126491.

Labrecque R, Fournier E, SIRARD MA. Transcriptome analysis of bovine oocytes from distinct follicle sizes: Insights from correlation network analysis. Mol Reprod Dev. 2016 Apr 29. PMID:27127921.

Khan DR, Fournier E, Dufort I, Richard FJ, Singh J, SIRARD MA. Meta-analysis of gene expression profiles in granulosa cells during folliculo genesis. Reproduction. 2016 Jun;151(6):R103-10 PMID:26980808.

Laskowski D, Sjunnesson Y, Humblot P, SIRARD MA, Andersson G, Gustafsson H, BågeR. Insulin exposure duringin vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential. Reprod Fertil Dev. 2016 Feb 29. PMID: 26922243.

Bergeron A, Guillemette C, SIRARD MA, Richard FJ. Active 3'-5'cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cells. Reprod Fertil Dev. 2016 Jan 4. PMID: 26724956.

Hosseini SM, Dufort I, Nieminen J, Moulavi F, Ghanaei HR, Hajian M, Jafarpour F, Forouzanfar M, Gourbai H, Shahverdi AH, Nasr-Esfahani MH, SIRARD MA. Epigenetic modification with trichostatin A does not correct specific errors of somatic cell nuclear transfer at the transcriptomic level; highlighting the non-random nature of oocyte-mediated reprogramming errors. BMC Genomics. 2016 Jan 4;17(1):16. PMID: 26725231

Hosseini SM, Dufort I, Caballero J, Moulavi F, Ghanaei HR, SIRARD MA.Transcriptome profiling of bovine inner cell mass and trophectoderm derived from in vivo generated blastocysts. BMC Dev Biol. 2015 Dec 18;15(1):49. PMID: 26681441.

Macaulay AD, Gilbert I, Scantland S, Fournier E, Ashkar F, Bastien A, Shojaei Saadi HA, Gagne D, SIRARD MA, Khandjian ÉW, Richard FJ, Hyttel P, Robert C Cumulus Cell Transcripts Transit to the Bovine Oocyte in Preparation for Maturation. Biol Reprod. 2015 Nov 19  PMID: 26586844.

de Oliveira LemeL, Dufort I, Spricigo JF, Braga TF, SIRARD MA, Franco MM,Dode MA. Effect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos. Theriogenology. 2015 Oct 22. PMID: 26553569

Salilew-Wondim D, Fournier E, Hoelker M, Saeed-Zidane M, Tholen E, Looft C, Neuhoff C, Besenfelder U, Havlicek V, Rings F, Gagné D, SIRARD MA, Robert C, A Shojaei Saadi H, Gad A, Schellander K, Tesfaye D. Genome-Wide DNA Methylation Patterns of Bovine Blastocysts Developed In Vivo from Embryos Completed Different. Stages of Development In Vitro. PLoS One. 2015 Nov 4;10(11): PMID: 26536655

Girard A, Dufort I, SIRARD MA. The effect of energy balance on the transcriptome of bovine granulosa cells at 60 days postpartum. Theriogenology. 2015 Jul 20.

Gagnon A, Khan DR, SIRARD MA, Girard CL, Laforest JP, Richard FJ. Effects of intramuscular administration of folic acid and vitamin B(12) on granulosa cells gene expression in postpartum dairy cows. J Dairy Sci. 2015 Aug19. doi: 10.3168/jds.2015-9623.

Guillemin N, Dufort I and SIRARD MA. Genetical Genomics Methodologyto IdentifyGeneticMarkers ofa Bovine FertilityPhenotype Based on CYP19A1 Gene Expression. Gene Expressionto Genetical Genomics 2015:8 9–18

Khan DR, Guillemette C, SIRARD MA, Richard FJ. Transcriptomic analysis of cyclic AMP response in bovine cumulus cells. Physiol Genomics. 2015 Jun 16

Labrecque R, Lodde V, Dieci C, TessaroI, Luciano AM, SIRARD MA.Chromatin remodelling and histone (m) RNA accumulation in bovine germinal vesicle oocytes. Mol Reprod Dev. 2015

Khan DR, Guillemette C, SIRARD MA, Richard FJ. Characterization of FSH signalling networks in bovine cumulus cells: a perspective on oocyte competence acquisition. Mol Hum Reprod. 2015

Girard A, Dufort I, Douville G, SIRARD MA. Global gene expression in granulosa cells of growing, plateau and atretic dominant follicles in cattle. Reprod Biol Endocrinol. 2015 ;13(1):10. Epub 2015 Mar 8.

Assidi M, Montag M, SIRARD MA. Use of both cumulus cells' transcriptomic markers and zona pellucida birefringence to select developmentally competent oocytes in human assisted reproductive technologies. BMC Genomics. 2015;16:6943.

Khan MI, Dias FC, Dufort I, Misra V, SIRARD MA, Singh J. Stable referencegenes in granulosa cells of bovine dominant follicles during follicular growth, FSH stimulation and maternal aging. Reprod Fertil Dev. 2014 Nov 27.

Bunel A, Jorssen EP, Merckx E, Leroy JL, Bols PE, SIRARD MA. Individual bovine in vitro embryo production and cumulus cell transcriptomic analysis to distinguish cumulus-oocyte complexes with high or low developmental potential. Theriogenology. 2015 Jan 15;83(2):228-37.

Dufort I, Robert C, SIRARD MA. Studying bovine early embryo transcriptome by microarray. Methods Mol Biol. 2015;1222:197-208.

Dias FC, Khan MI, Adams GP, SIRARD MA, Singh J. Granulosa cell function and oocyte competence: Super-follicles, super-moms and super-stimulation in cattle. Anim Reprod Sci. 2014 Jul 24. pii: S0378-4320(14)00232-2.

Macaulay AD, Gilbert I, Caballero J, Barreto R, Fournier E, Tossou P, SIRARD MA, Clarke HJ, Khandjian EW, Richard FJ, Hyttel P, Robert C. The Gametic Synapse; RNA Transfer to the Bovine Oocyte. Biol Reprod. 2014 Aug 20. [Epub ahead of print] PubMed PMID: 25143353.

Dobbs KB, Gagné D, Fournier E, Dufort I, Robert C, Block J, SIRARD MA, Bonilla L, Ealy AD, Loureiro B, Hansen PJ. Sexual Dimorphism in Developmental Programming of the Bovine Preimplantation Embryo Caused by Colony Stimulating Factor 2. Biol Reprod. 2014 Jul 30..

SIRARD MA Toward building the cow folliculome. Anim Reprod Sci. 2014 Jun 30.

Golini VE, Stradaioli G, SIRARD MA. Transcriptome analysis of bovine granulosa cells of preovulatory follicles harvested 30, 60, 90, and 120 days postpartum. Theriogenology. 2014 Jun 4.

Douville G, SIRARD MA. Changes in granulosa cells gene expression associated with growth, plateau and atretic phases in medium bovine follicles. J Ovarian Res. 2014 May 7;7:50.

Shojaei Saadi HA, O Doherty AM, Gagné D, Fournier E, Grant JR, SIRARD MA, Robert C. An integrated platform for bovine DNA methylome analysis suitable for small samples. BMC Genomics. 2014 Jun 9;15(1):451.

Labrecque R, Vigneault C, Blondin P, SIRARD MA.. Gene expression analysis of bovine oocytes at optimal coasting time combined with GnRH antagonist during the no-FSH period. Theriogenology. 2014 May;81(8):1092-100 2014 Jan 31.

Pagé-Larivière F, SIRARD MA. Spatiotemporal expression of DNA demethylation enzymes and histone demethylases in bovine embryos. Cell Reprogram. 2014Feb;16(1):40-53.

Cagnone G, SIRARD MA. The impact of exposure to serum lipids during in vitro culture on the transcriptome of bovine blastocysts. Theriogenology. 2014 Mar 15;81(5):712-22

Van Hoeck V, Rizos D, Gutierrez-Adan A, Pintelon I, Jorssen E, Dufort I, SIRARD MA, Verlaet A, Hermans N, Bols PE, Leroy JL. Interaction between differential gene expression profile and phenotype in bovine blastocysts originating from oocytes exposed to elevated non-esterified fatty acid concentrations. Reprod Fertil Dev. 2013 Dec 23.

Orozco-Lucero E, Dufort I, Robert C, SIRARD MA. Rapidly cleaving bovine two-cell embryos have better developmental potential and distinctive mRNA pattern. Mol Reprod Dev. 2013 Nov 7.

Labrecque R, SIRARD MA. The study of mammalian oocyte competence by transcriptome analysis: progress and challenges. Mol Hum Reprod. 2013 Nov 27.

Gohin M, Fournier E, Dufort I, SIRARD MA. Discovery, identification and sequence analysis of RNAs selected for very short or long poly A tail in immature bovine oocytes. Mol Hum Reprod. 2013 Dec 2.

Assidi M, Richard FJ, SIRARD MA. FSH in vitro versus LH in vivo: similar genomic effects on the cumulus. J Ovarian Res. 2013 Sep 25;6(1):68.

Bunel A, Nivet AL, Blondin P, Vigneault C, Richard FJ, SIRARD MA. Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes. Reprod Fertil Dev. 2013 Jul 5. [Epub ahead of print]

de Montera B, Fournier E, Shojaei Saadi HA, Gagné D, Laflamme I, Blondin P, SIRARD MA, Robert C. Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine. BMC Genomics. 2013 Jun 18;14:406. [Epub ahead of print]

Dias FC, Khan MI, SIRARD MA, Adams GP, Singh J. Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle. Reproduction. 2013 Jun 6. [Epub ahead of print]

Nivet AL, Vigneault C, Blondin P, SIRARD MA. Changes in granulosa cells gene expression associated with increased oocyte competence in bovine. Reproduction. 2013

Labrecque R, Vigneault C, Blondin P, SIRARD MA. Gene expression analysis of bovine oocytes with high developmental competence obtained from FSH-stimulated animals. Mol Repr Dev. 2013

Cagnone GL, SIRARD MA. Transcriptomic signature to oxidative stress exposure at the time of embryonic genome activation in bovine blastocysts. Mol Reprod Dev 2013

Sylvestre EL, Robert C, Pennetier S, Labrecque R, Gilbert I, Dufort I, Léveillé MC, SIRARD MA. Evolutionary conservation of the oocyte transcriptome among vertebrates and its implications for understanding human reproductive function. Mol Hum Reprod. 2013 Jan 21.

 

Other contributions (Book chapters)

 

SIRARD MA. The ovarian follicle of cows as a model for humanIn: Animal Models and HumanReproduction: Cell and Molecular Approaches with Reference to Human Reproduction. HeideSchatten (Editor), Gheorghe M. Constantinescu (Editor) ISBN: 978-1-118-88160-6.600 pagesMarch 2017, Wiley-Blackwell

Dufort I, Robert C and SIRARD MA. "Studying bovine early embryo transcriptome by microarray", Nuclear Reprogramming in "Methods in Molecular Biology", 1-16, Humana Press Editors: Alice JOUNEAU, Hélène JAMMES, Dr Nathalie BEAUJEAN 2014

SIRARD MA, Gilbert I. Omics for oocyte selection Oocyte pathology " Biology and Pathology of the Oocyte."   Edited by alan Trounson. Cambridge University Press 2013 297-305

SIRARD MA Screening for oocyte competence. In “Oocyte Physiology and Development in Domestic Animals”. R Krisher ed. Wiley-Blackwell, John Wiley & Sons. 2011 184-198. 2013

Assidi M and SIRARD MA . Cumulus cells' gene expression as a manifestation of oocyte quality.In: Mammalian oogenesis. Eds: G. Coticchio, L. De Santis, D. Albertini. Elsevier. 2012

SIRARD MA, Mammalian embryonic genome activation in Reproduction in Domestic Ruminants VII. Smith, Lucy, Pate and Spencer (Eds). Nottingham University Press. 2011. 212-225.

 

Awards:

Prix Léo Pariseau 2014. Prix annuel de L’AFAS en sciences de la Santé