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  NGévry

Nicolas Gévry, PhD

Professeur agrégé

Université de Sherbrooke

Département de Biologie

2500, boulevard de l’Université

Courriel: This email address is being protected from spambots. You need JavaScript enabled to view it.

Téléphone: 819-821-8000 ext.: 66013

Fax: 819-821-8049

 

Field of research:

Transcriptional regulatory mechanisms and chromatin remodelling by nuclear receptors in the endocrine system.

 

Recent results:

Nuclear receptors make up one of the largest transcription factor family, with 48 members identified to date. Nuclear receptor driven transcriptional activation is generally controlled by ligand binding of lipophyllic molecules such as hormones or other metabolites (eg. fatty acids or oxysterol) to a specific nuclear receptor. However, some receptors have no known ligand and are activated through alternative mechanisms. These nuclear receptors are classified as orphan receptors. Generally, nuclear receptors are complexed with other effector molecules, co-activator or co-repressors, within the cells. Typically, ligand biding or phosphorylation will change the conformation of the nuclear receptor and activate it. This conformational change alters co-repressor and co-activator affinity to the nuclear factor and favours the recruitment of co-activators. These co-repressors and co-activators, often composed of multiple proteins, will modify the accessibility of the transcriptional machinery to targeted promoters and inhibit or favour transcription initiation.

Our knowledge on nuclear receptor signalling has grown in the last decade with the understanding that genetic regulation by nuclear receptors is infinitely more complex than the simple binding of DNA by the liganded receptors. Genes are regulated in a dynamic manner, where the interactions between co-factors, nuclear receptors and the chromatin environment also play a fundamental part in signal transduction. Since nuclear receptors are the switches integrating endocrine signals at the cellular level in health and disease, understanding their mechanisms of action and function in various physiological circumstances is vital to the development of future therapies for diseases such as breast cancer or infertility.

Specific research interests: 

  • To study the role of the chromatin structure and remodelling complexes in activation and repression of genes regulated by the estrogen receptor in health and disease.
  • To study the mechanism of gene transcription and chromatin remodelling implicated in the maintenance of the ovarian function.

 

Laboratory members:

Name

Position

Email Address

Élaine Beaulieu

Research Professionnal

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Stéphanie Bianco

Postdoctoral Fellow

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Jean-Phillipe Couture

Postdoctoral Fellow

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Mylène Brunelle

Ph.D. student

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Maïka Jangal

Ph.D. student

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Alexei Nordell-Markovits

Ph.D. student

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Audrey Allard

M.Sc. student 

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List of publications:

  1. Nordell Markovits A, Joly-Beauparlant C, Toupin D, Droit A, Shengrui W, Gévry N. (2013) NGS++, a library for rapid prototyping of epigenomics analysis programs. Bioinformatics (In press)
  2. Nolet G, Daviau A, Blouin R and Gévry N. (2012) The p400/Brd8 chromatin remodelling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPARgamma target genes. Endocrinology, Dec;153(12):5796-808.
  3. Magnani L, Brunelle M, Gévry N and Lupien M. (2012) Chromatin Landscape and Endocrine Response in Breast Cancer. Epigenomics, Dec;4(6):675-83.
  4. Brunelle M, Gévry N, Lupien M. (2012) Les mutations épigénétiques spécifiques aux enhancers typifient le cancer colorectal. Médecine/Sciences, Nov;28(11):929-31. 
  5. Beshiri M, Holmes K, Richter W, Hess S, Islam A, Yan Q, Plante L, Gévry N, Lopez-Bigas N, Kaelin W and Benevolenskaya E. (2012) Demethylation by KDM5A Contributes to Repression of Cell Cycle Genes during Differentiation. Proceedings of the National Academy of Sciences, Nov 6;109(45):18499-504.
  6. Bianco S and Gévry N. (2012) Chromatin determinants implicated in hormonal therapy responsiveness. Transcription, Jul-Aug;3(4):165-70.
  7. Svotelis, A., Bianco, S., Madore, J., Huppe, G., Nordell-Markovits, A., Mes-Masson, A.M., and Gevry, N. 2011. H3K27 demethylation by JMJD3 at a poised enhancer of anti-apoptotic gene BCL2 determines ERalpha ligand dependency. The EMBO Journal 12;30 (19):3947-61
  8. Svotelis#, A., Gevry, N.*#, Grondin, G., and Gaudreau, L*. 2010. H2A.Z overexpression promotes cellular proliferation of breast cancer cells. Cell Cycle 9(2): 364-370. (*auteur co-correspondant et #co-premier).
  9. Gevry, N., Hardy, S., Jacques, P.E., Laflamme, L., Svotelis, A., Robert, F., and Gaudreau, L. (2009). Histone H2A.Z is essential for estrogen receptor signaling. Genes & Development 23(13): 1522-1533.
  10. Hardy, S., Jacques, P.E., Gevry, N., Forest, A., Fortin, M.E., Laflamme, L., Gaudreau, L., and Robert, F. (2009). The euchromatic and heterochromatic landscapes are shaped by antagonizing effects of transcription on H2A.Z deposition. PLoS Genetics 5(10): e1000687.
  11. Svotelis, A., Gevry, N., and Gaudreau, L. (2009). Regulation of gene expression and cellular proliferation by histone H2A.Z. Biochemistry and Cell Biology 87(1): 179-188.
  12. Gevry, N., Svotelis, A., Larochelle, M., and Gaudreau, L. (2009). Nucleosome mapping. Methods in Molecular Biology 543: 281-291.
  13. Svotelis, A., Gevry, N., and Gaudreau, L. (2009). Chromatin immunoprecipitation in mammalian cells. Methods in Molecular Biology 543: 243-251.
  14. Gevry, N., Schoonjans, K., Guay, F., and Murphy, B.D. (2008). Cholesterol supply and SREBPs modulate transcription of the Niemann-Pick C-1 gene in steroidogenic tissues. Journal of Lipid Research 49(5): 1024-1033.
  15. Gevry, N., Chan, H.M., Laflamme, L., Livingston, D.M., and Gaudreau, L. (2007). p21 transcription is regulated by differential localization of histone H2A.Z. Genes & Development 21(15): 1869-1881.
  16. Lopes, F.L., Desmarais, J., Ledoux, S., Gevry, N.Y., Lefevre, P., and Murphy, B.D. (2006). Transcriptional regulation of uterine vascular endothelial growth factor during early gestation in a carnivore model, Mustela vison. The Journal of Biological Chemistry 281(34): 24602-24611.
  17. Guillemette, B., Bataille, A.R., Gevry, N., Adam, M., Blanchette, M., Robert, F., and Gaudreau, L. (2005). Variant histone H2A.Z is globally localized to the promoters of inactive yeast genes and regulates nucleosome positioning. PLoS Biology 3(12): e384.
  18. Kim, M.Y., Mauro, S., Gevry, N., Lis, J.T., and Kraus, W.L. (2004). NAD+-dependent modulation of chromatin structure and transcription by nucleosome binding properties of PARP-1. Cell 119(6): 803-814.
  19. Gevry, N.Y., Lopes, F.L., Ledoux, S., and Murphy, B.D. (2004). Aberrant intracellular cholesterol transport disrupts pituitary and ovarian function. Molecular Endocrinology 18(7): 1778-1786.
  20. Gevry, N., Lalli, E., Sassone-Corsi, P., and Murphy, B.D. (2003). Regulation of niemann-pick c1 gene expression by the 3'5'-cyclic adenosine monophosphate pathway in steroidogenic cells. Molecular Endocrinology 17(4): 704-715.
  21. Lopes, F.L., Desmarais, J., Gevry, N.Y., Ledoux, S., and Murphy, B.D. (2003). Expression of vascular endothelial growth factor isoforms and receptors Flt-1 and KDR during the peri-implantation period in the mink, Mustela vison. Biology of Reproduction 68(5): 1926-1933.
  22. Ruiz-Cortes, Z.T., Martel-Kennes, Y., Gevry, N.Y., Downey, B.R., Palin, M.F., and Murphy, B.D. (2003). Biphasic effects of leptin in porcine granulosa cells. Biology of Reproduction 68(3): 789-796.
  23. Gevry, N., Lacroix, D., Song, J.H., Pescador, N., Dobias, M., and Murphy, B.D. (2002). Porcine Niemann Pick-C1 protein is expressed in steroidogenic tissues and modulated by cAMP. Endocrinology 143(2): 708-716.
  24. Gevry, N.Y. and Murphy, B.D. (2002). The role and regulation of the Niemann-Pick C1 gene in adrenal steroidogenesis. Endocrine Research 28(4): 403-412.
  25. Murphy, B.D., Gevry, N., Ruiz-Cortes, T., Cote, F., Downey, B.R., and Sirois, J. (2001). Formation and early development of the corpus luteum in pigs. Reproduction 58: 47-63.