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   PL    

Pierre Leclerc, Ph.D.

Professor

Université Laval

Department: Obstétrique et gynécologie

Centre de recherche en biologie de la reproduction

Axe reproduction, santé périnatale et santé de l’enfant

Local T3-67, Centre de recherche du CHUQ-CHUL

2705, boul. Laurier, Québec

G1V 4G2

Tel.: (418) 525-4444, extension 46267

Fax: (418) 654-2783

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Field of research: 

The main objective of my research program is to better understand the mechanisms involved in the interactions between gametes, particularly from the sperm point-of-view. To fertilize the egg, sperm must undergo a numerous changes at the membrane and intracellular levels. Therefore, the properties of its plasma and acrosomal membranes will be affected, as well as the activity of several enzymes and the gamete’s ionic content. This post-ejaculatory phenomenon called capacitation normally occurs in the female genital tract during the sperm transit to the site of fertilization. Another aspect studied, although connected, is to directly study the mechanisms of sperm recognition and binding at the surface of the egg’s zona pellucida. To this matter, the role of the sperm protein SPAM1 will be investigated. A better understanding of the mechanisms involved in the sperm acquisition of fertilizing ability and in sperm/egg interactions is important in farm animal productions where profitability often depends on the efficiency of the animal reproductive capacity. In humans, sperm/egg interactions are of particular interest for diagnosis/treatment of infertility, or for the development of new contraceptive methods. Various projects are underway in my laboratory.

  • Investigation of the signal transduction pathways during sperm capacitation and acrosomal exocytosis;
  • Identification and characterization of sperm tyrosine kinases;
  • Regulation of intracellular pH and Ca2+ concentration;
  • Involvement of the oviduct cells on the maintenance of sperm viability;
  • Role of the oviduct on the sperm’s acquisition of fertilizing ability;
  • Improvement of sperm viability and fertilizing ability upon cryopreservation;
  • Role of SPAM1 in sperm/egg interactions

 

Laboratory Members: 

Name

Position

Email Address

Serge Goupil

Research professional

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Catherine Lachance

Research professional

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Ahmadréza Sédighi

PhD student

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Loïze Maréchal

M.Sc student

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List of Publications (last 5 years):

Lachance, C., Goupil, S., LECLERC, P. (2013) Stattic V, a STAT3 inhibitor, affects human spermatozoa through regulation of mitochondrial activity. J Cell Physiol 228: 704-713.

Lachance, C., LECLERC, P. (2011) Mediators of the JAK/STAT signalling pathway in human spermatozoa. Biol. Reprod. (10.1095/biolreprod.111.092379).

Goupil, S., La Salle, S., Trasler, J.M., Bordeleau, L.-J., LECLERC, P. (2011) Developmental expression of src-related tyrosine kinases in the mouse testis. J. Androl. 32: 95-110.

Morin, G., Sullivan, R. Laflamme, I., Robert, C., LECLERC, P. (2010) SPAM1 isoforms from two tissue origins are differentially localized within ejaculated bull sperm membranes and have different roles during fertilization. Biol. Reprod. 82: 271-281.

Lachance, C., Fortier, M., Thimon, V., Sullivan, R., Bailey, J.L., LECLERC, P. (2010) Localization of Hsp60 and Grp78 in the human testis, epididymis and mature spermatozoa. Int. J. Androl. 33(1): 33-44.

Pons-Rejraji, H., Bailey, J.L., LECLERC, P. (2009) Modulation of bovine sperm signalling pathways: correlation between intracellular parameters and sperm capacitation and acrosome exocytosis. Reprod Fert Dev 21: 511-524.

Pons-Rejraji, H., Bailey, J.L., LECLERC, P. (2009) Cryopreservation affects bovine sperm intracellular parameters associated with capacitation and acrosome exocytosis. Reprod Fert Dev 21: 525-537.

Lawson, C., Goupil, S., LECLERC, P. (2008) Increased activity of human sperm tyrosine kinase src by adenosine 3’, 5’-monophosphate cyclic and Ca2+. Biol. Reprod. 79: 657-666.

Bordeleau, L.-J., LECLERC, P. (2008) Expression of hck-tr, a truncated form of the src-related tyrosine kinase hck, in bovine spermatozoa and testis. Mol. Reprod. Dev. 75: 828-837.

Lachance, C., Bailey, J.L., LECLERC, P. (2007) Expression of hsp60 and grp78 in the human endometrium and oviduct, and their effect on sperm functions. Hum. Reprod. 22: 2606-2614.

Lawson, C., Dorval, V., Goupil, S., LECLERC, P. (2007) Identification and localisation of SERCA 2 isoforms in mammalian sperm. Mol. Hum. Reprod. 13: 307-316.

Lalancette, C., Faure, R., LECLERC, P. (2006) Identification of the proteins present in the bull sperm cytosolic fraction enriched in tyrosine kinase activity: a proteomic approach. Proteomics 6: 4523-4540.

Lalancette, C., Bordeleau, L.-J., Faure, R.L., LECLERC, P. (2006) Bull testicular haploid germ cells express a messenger encoding for a truncated form of the protein tyrosine kinase HCK. Mol. Reprod. Dev. 73: 520-530.