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RS

Robert Sullivan

Professeur titulaire

Département d’obstétrique-gynécologie

Université Laval

Centre de recherche du CHUQ (CHUL)

Local T3-67

2705, boul. Laurier

Québec, Qc

Canada, G1V 4G2

Tél : (418) 525-4444, poste 46104

Fax : (418) 654-2765

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Field of research: 

  • Infertilité et contraception masculines
  • Fécondation
  • Spermatologie
  • Maturation des spermatozoïdes
  • Tractus reproducteur mâle
  • Épididyme
  • Vasectomie, vasovasostomie

 

Resume of recent results :

Bien que différencié, le spermatozoïde qui quitte le testicule est incapable de se mouvoir et de féconder. Pour se faire il doit traverser l’épididyme, un long tubule circonvolué accolé à la paroi du testicule. Sous l’effet des androgènes, l’épithélium de l’épididyme sécrète des protéines dans la lumière épididymaire qui, en interagissant avec le spermatozoïde vont lui permettre d’acquérir sa fonctionnalité. L’expression des gènes le long de l’épididyme est finement régulée ce qui fait en sorte que la composition du milieu intraluminal de l’épididyme varie le long de l’organe. Les modifications encourues par le spermatozoïde au cours de sa maturation épididymaire sont donc séquentielles. Nous nous intéressons à ces modifications permettant au spermatozoïde de devenir fonctionnel. Plus spécifiquement, nous avons identifié une famille de protéines épididymaires acquises par le spermatozoïde lors de sa maturation. Par des techniques de biologie moléculaire nous étudions le rôle de ces protéines dans le processus de fécondation. Nous avons d’autre part émis l’hypothèse de ces protéines sont des instruments diagnostic de l’infertilité masculine et qu’elles représentent des cibles pour développer une contraception masculine vaccinale. En parallèle, nous tentons d’élucider les mécanismes d’ancrage des protéines épididymaires aux spermatozoïdes et nous nous intéressons aux conséquences de la vasectomie sur la physiologie de l’épididyme et la réversibilité des séquelles encourues par l’épididyme sous vasectomie suite à la vasovasostomie.

 

List of laboratory members:

Name

Position

Email Address

Christine Légaré

Research Professionnal

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Gilles Frenette

Research Professionnal

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Olivier D’Amours

PhD student

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Ayodélé Akintayo

PhD student

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List of publications (since 2005):

Morin G, Lalancette C, Sullivan R, Leclerc P. Identification of the bull p80 protein as a PH-20 orhtolog and its modification during the epididymal transit. Molec Reprod Dev, 71: 523-534, 2005.

Frenette G, Légaré C, Saez F, Sullivan R. Macrophage migration Inhibitory Factor in the human epididymis and semen. Molec Hum Reprod, 11: 575-582, 2005.

Sullivan R, Frenette G, Légaré C. Sécrétions apocrines et glandes annexes. Andrologie, 15: 35-40, 2005.

Dubé E, Légaré C, Sullivan R. Contraceptive responses of female hamsters immunized with recombinant sperm protein P26h. Contraception, 72: 459-467, 2005.

Sullivan R, Saez F, Girouard J, Frenette G Role of exosomes in sperm maturation during the transit along the male reproductive tract Blood Cells, Molec, & Dis, 35: 1-10, 2005

Frenette G, Thabet M, Sullivan R. Polyol pathway in human semen and epididymis J Androl, 27: 233-239, 2006.

Sullivan R, Légaré C, Villeneuve M, Foliguet B, Bissonnette F. Levels of P34H, an epididymal originating sperm protein, as a predictor of conventional In Vitro Fertilization outcome. Fertil Steril, 85: 1557-1559, 2006.

Desrosiers P, Légaré C, Leclerc P, Sullivan R. Membranous and structural damages occurring during cryoperservation of human sperm: time-related events? Fertil Steril, 85: 1744-1752, 2006.

Légaré C, Thabet M, Gatti JL, Sullivan R. HE1/NPC2 status in human reproductive tract and ejaculated spermatozoa: consequence of vasectomy. Molec Hum Reprod, 12 : 461-468, 2006.

Frenette G, Girouard J, Sullivan R Comparison between epididymosomes collected in the intraluminal compartment of the bovine caput and cauda epididymidis. Biol Reprod, 75: 885-890, 2006.

Lefebvre J, Fan J, Chevalier S, Sullivan R, Carmona E, Manjunath P. Genomic structure and tissue-specific expression of human and mouse genes encoding homologs of the major bovine seminal plasma proteins. Molec Hum Reprod, 13: 45-53, 2007.

Moskovtsev SI, Jarvi K, Légaré C, Sullivan R, Mullen JBM. Epididymal P34H protein detection in men evaluated for infertility. Fertil Steril 88 : 1455-1457, 2007.

Sullivan R, Frenette G, Girouard J. Epididymosomes are involved in the acquisition of new sperm proteins during the epididymal transit. Asian J Androl 9: 483-492, 2007.

Sullivan R, Légaré C, Thimon V. Effects of vasectomy on protein secretion and sperm maturation along the human epididymis. ANHP-ANIR 9: 43-49, 2007.

Thimon V, Koukoui O, Calvo E, Sullivan R. Region-specific gene expression profiling along the human epididymis. Molec Hum Reprod 13: 691-704, 2007.

Thimon V, Calvo E, Koukoui O, Légaré C, Sullivan R. Effects of vasectomy on gene expression profiling along the human epididymis. Biol Reprod, 79: 262-273, 2008.

Thimon V, Frenette G, Saez F, Thabet M, Sullivan R. Protein composition of human epididymosomes collected during surgical vasectomy reversal: a proteomic and genomic approach. Human Reproduction, 23: 1698-1707, 2008

Reyes-Moreno C, Laflamme J, Frenette G, Sirard MA, Sullivan R. Spermatozoa regulate cell proliferation and protein secretion of epididymal cells cultured at two different temperatures. Molec Reprod Dev 75: 512-520, 2008.

Girouard J, Frenette G, Sullivan R. Seminal Plasma Proteins Regulate the Association of Lipids and Proteins Within Detergent-Resistant Membrane Domains of Bovine Spermatozoa. Biol Reprod, 78: 921-931, 2008.

Sasseville M, Gagnon MC, Guillemette C, Sullivan R, Gilchrist RB, Richard FJ Regulation of gap junction in cumulus-oocyte complexes: Contributions of granulosa cell contact, gonadotropins and lipid rafts. Molec Endocrinol, 23: 700-710, 2009.

Lachance C, Fortier M, Thimon V. Sullivan R, Bailey JL, Leclerc P Localization of HSP60 and GRP78 in the human testis, epididymis and mature spermatozoa. Int J Androl, 32: 33-44, 2009.

Frenette G, Leclerc P, Sullivan R Estrogen sulfotransferase is highly expressed along the bovine epididymis and is secreted in the intraluminal compartment. J Androl, 30: 580-589-2009.

Girouard J, Frenette G, Sullivan R. Compartimentalization of proteins in epididymosomes coordinates the association of epididymal proteins with the different functional structures of bovine spermatozoa. Biol Reprod, 80: 965-972, 2009.

Morin G, Sullivan R, Leclerc P SPAM1 isoforms from two different origins ore differentially localized within ejaculated bovine sperm membranes and have different roles during fertilization. Biol Reprod, 82: 271-281, 2010.

D’Amours O, Frenette G, Fortier M, Leclerc P, Sullivan R Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes Reproduction, 139: 545-556, 2010.

Legare C, Boudreau L, Thabet M, Sullivan R Vasectomy affects cysteine-rich secretory proteins1 (CRISP1) expression along the human epididymis and its association with ejaculated spermatozoa of vasovasostomized men. J Androl, 31: 573-583, 2010.

Frenette G, Girouard J, D’Amours O, Sullivan R Characterization of two distinct populations of epididymosomes collected in the intraluminal compartment of the bovine cauda epididymidis. Biology of Reproduction, 83: 473-480, 2010.

Caballero J, Frenette G, Sullivan R Post-testicular sperm maturational changes in the bull: important role of the epididymosomes and prostasomes. Ver Med Int. 2010 Oct 13; 2011: 757194.

Girouard J, Frenette G, Sullivan R. Comparative proteome an lipid profiles of bovine epididymosomes collected in the intraluminal compartment of the caput and cauda epididymis. Intern J Androl, 34: e475-e486, 2011.

Lessard C, Siquiera LS, D’Amours O, Sullivan R, Leclerc P, Palmer C. First bovine model to study the capacitation process. Theriogenology, 76: 891-899, 2011.

Sullivan R. Légaré C, Thabet M, Thimon V. Gene expression in the epididymis of normal and vasectomized men: what can we learn about human sperm maturation? J Androl, 32: 686-697, 2011.

Khatri P, Frenette G, Sullivan R, Hoffmann B Schuler G. Expression of SULT1E1 protein in bovine placentomes: evidence for localization in uninucleated trophobast cells. Placenta, 32: 431-440, 2011.

Caballero J, Frenette G, D’Amours O, Belleannée C, Robert C, Sullivan R. Bovine sperm Raft membrane associated Glioma Pathogenesis-Related 1-like Protein 1 (GliPr1L1) is modified during the epididymal transit and is potentially involved in sperm binding to the zona pellucida. J Cell Physiol, 227: 3876-3886, 2012.

Caballero J, Frenette G, D’Amours O, Oko R, Dufour M, Sullivan R. ATP-binding cassette transporter G2 activity in the bovine spermatozoa is modulated along the epididymal duct and at ejaculation. Biol Reprod, 86: 1-11, 2012.

D’Amours O, Frenette G, Bordeleau LJ, Leclerc P, Blondin P, Sullivan R. Binder of sperm 1 (BSP1) and epididymal sperm binding protein 1 (ELSPBP1) are associated with different bull sperm subpopulations. Reproduction, 143:759-71, 2012.

Belleannée C, Thimon V, Sullivan R. Region-specific gene expression in the epididymis. Cell Tissues Res, Ahead of print, 2012 March 18.

Belleannée C, Calvo E, Thimon V, Cyr D, Légaré C, Garneau L, Sullivan R. Role of microRNAs on the pattern of gene expression in the different segments of human epididymis. PLoS One. 2012: 7(4):e34996. Epub 2012 Apr 12.

D’Amours O, Frenette G, Bordeleau LJ, Allard N, Leclerc P, Blondin P, Sullivan R. Epididymosomes transfer Epididymal Sperm Binding Protein 1 (ELSPBP1) to dead spermatozoa during epididymal maturation. Biol Reprod, 87: 1-11, 2012.

Larose J, Laflamme J, Côté I, Lapointe J, Frenette G, Sullivan R, Bilodeau JF. The polyol pathway in the bovine oviduct. Molec Reprod Dev, 79: 603-612, 2012.

Caballero JN, Frenette G, Belleannée C, Sullivan R. CD9-positive microvesicles mediate the transfer of molecules to bovine spermatozoa during epididymal transit. Plos One, In press, april 2013.

Belleannée C, Calvo E, Légaré C, Thimon V, Sullivan R. MicroRNA signature is altered in both human epididymis and seminal microvesicles following vasectomy. Hum Reprod, ehead of print, March 28th, 2013.

Sullivan R, Saez F. Epididymosomes, prostasomes and liposomes; their roles in mammalian male reproductive physiology. Reproduction, first published 23 April 2013.